%0 Journal Article
%T Simultaneous ultra-high frequency photoacoustic microscopy and photoacoustic radiometry of zebrafish larvae in vivo
%A Michael C. Kolios
%A Michael J. Moore
%A Suzan El-Rass
%A Xiao-Yan Wen
%A Yongliang Xiao
%A Youdong Wang
%J Archive of "Photoacoustics".
%D 2018
%R 10.1016/j.pacs.2018.08.004
%X With their optically transparent appearance, zebrafish larvae are readily imaged with optical-resolution photoacoustic (PA) microscopy (OR-PAM). Previous OR-PAM studies have mapped endogenous chromophores (e.g. melanin and hemoglobin) within larvae; however, anatomical features cannot be imaged with OR-PAM alone due to insufficient optical absorption. We have previously reported on the photoacoustic radiometry (PAR) technique, which can be used simultaneously with OR-PAM to generate images dependent upon the optical attenuation properties of a sample. Here we demonstrate application of the duplex PAR/PA technique for label-free imaging of the anatomy and vasculature of zebrafish larvae in vivo at 200 and 400£¿MHz ultrasound detection frequencies. We then use the technique to assess the effects of anti-angiogenic drugs on the development of the larval vasculature. Our results demonstrate the effectiveness of simultaneous PAR/PA for acquiring anatomical images of optically transparent samples in vivo, and its potential applications in assessing drug efficacy and embryonic development
%K Label-free
%K Vasculature
%K Angiogenesis
%K Photoacoustic radiometry
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139000/