%0 Journal Article
%T Characterization and epitope identification of the T cell response in non-allergic individuals exposed to mouse allergen
%A Alba Grifoni
%A Alessandro Sette
%A Bjoern Peters
%A Giovanni Birrueta
%A John Pham
%A Luise Westernberg
%A Ricardo da Silva Antunes
%A V¨¦ronique Schulten
%J Archive of "The World Allergy Organization Journal".
%D 2019
%R 10.1016/j.waojou.2019.100026
%X Exposure to airborne allergens is a frequent trigger of respiratory allergy and asthma in atopic individuals. While allergic patients suffer hypersensitivity reactions to these allergens, non-allergic individuals do not exhibit clinical symptoms despite environmental exposure to these ubiquitous allergen sources. The aim of this study was to characterize T cell responses in non-allergic laboratory workers, who are heavily exposed to mice allergens (Exposed Non-Allergics, ENA) and compare this data to previously published T cell responses measured in mouse (MO)-allergic patients. METHODS: Peripheral mononuclear cells (PBMC) from ENA subjects were expanded for 2 weeks in vitro with mouse urine extract and screened for IFN¦Ã and IL-5 cytokine production in response to mouse antigen-derived peptides by ELISPOT. Ex vivo T cell reactivity in the ENA cohort was performed after 6hr stimulation with peptide pools by intracellular staining of CD154
%K Mouse allergy
%K T cells
%K Cytokines
%K T cell epitopes
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479169/