%0 Journal Article
%T Allele-specific expression and alternative splicing in horseˇÁdonkey and cattleˇÁyak hybrids
%A Guang-Xian Zhou
%A Jing-Tao Xu
%A Jun-Jie Shao
%A Lei Wang
%A Ming Li
%A Ni-Ni Wang
%A Shan Gao
%A Wei-Dong Deng
%A Wei-Huang Chen
%A Wen Wang
%A Wen-Jing Shen
%A Yu Jiang
%A Yu Wang
%A Yu-Lin Chen
%A Yue Zhao
%J Archive of "Zoological Research".
%D 2019
%R 10.24272/j.issn.2095-8137.2019.042
%X Divergence of gene expression and alternative splicing is a crucial driving force in the evolution of species; to date, however the molecular mechanism remains unclear. Hybrids of closely related species provide a suitable model to analyze allele-specific expression (ASE) and allele-specific alternative splicing (ASS). Analysis of ASE and ASS can uncover the differences in cis-regulatory elements between closely related species, while eliminating interference of trans-regulatory elements. Here, we provide a detailed characterization of ASE and ASS from 19 and 10 transcriptome datasets across five tissues from reciprocal-cross hybrids of horseˇÁdonkey (mule/hinny) and cattleˇÁyak (dzo), respectively. Results showed that 4.8%¨C8.7% and 10.8%¨C16.7% of genes exhibited ASE and ASS, respectively. Notably, lncRNAs and pseudogenes were more likely to show ASE than protein-coding genes. In addition, genes showing ASE and ASS in mule/hinny were found to be involved in the regulation of muscle strength, whereas those of dzo were involved in high-altitude adaptation. In conclusion, our study demonstrated that exploration of genes showing ASE and ASS in hybrids of closely related species is feasible for species evolution research
%K Allele-specific alternative splicing
%K Allele-specific expression
%K Cis-regulatory elements
%K Hybrid species
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680129/