%0 Journal Article
%T Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography
%A Byoung Ju Kim
%A Byoung-Hyun Min
%A Byung Hyune Choi
%A Hyun Ju Oh
%A Long Hao Jin
%A Xiang Yun Yin
%A Young Jick Kim
%J Archive of "Tissue Engineering and Regenerative Medicine".
%D 2018
%R 10.1007/s13770-018-0117-y
%X Optimization of the incubation and washing time of Hexabrix 320 for engineered cartilages. A Tissue density of a cartilage tissue was measured using ¦ÌCT analysis at indicated time points until 120 min after incubation with Hexabrix 320 (n£¿=£¿4, mean£¿¡À£¿SD). The result indicated that 20¨C30 min of Hexabrix 320 incubation was sufficient to reach an equilibration (*p£¿<£¿0.05 vs 20 min and #p£¿<£¿0.05 vs 30 min samples). B After incubation with Hexabrix 320 for 20 min, the cartilage tissue was incubated with washing medium until 30 min. The tissue density was measured using ¦ÌCT at 0, 10, 20 and 30 min after washing with the washing medium changed every time. Decline of tissue density by 86% was observed at 30 min (*p£¿<£¿0.05 vs 30 min samples). No Hex represents a tissue intensity without Hexabrix treatmen
%K Micro-CT (¦ÌCT)
%K Glycosaminoglycans (GAGs)
%K Hexabrix 320
%K Cartilage
%K Tissue engineering
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171677/