%0 Journal Article
%T Surface Display of Antigen Protein VP8* of Porcine Rotavirus on Bacillus Subtilis Spores Using CotB as a Fusion Partner
%A Abdul Khalique
%A Bo Jing
%A Dong Zeng
%A Dongmei Zhang
%A Jiajun Li
%A Jianzhen Li
%A Jie Feng
%A Kangcheng Pan
%A Miao Yang
%A Qihui Luo
%A Wanqiang Li
%A Xueqin Ni
%A Zhenhua Wang
%J Molecules | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/molecules24203793
%X Porcine rotavirus is a major cause of acute viral gastroenteritis in suckling piglets, and vaccination is considered to be an effective measure to control these infections. The development of a live mucosal vaccine using Bacillus subtilis spores as an antigen delivery vehicle is a convenient and attractive vaccination strategy against porcine rotavirus. In this study, a shuttle vector was constructed for the spore surface display of the spike protein VP8* from porcine rotavirus (the genotype was G5P[7]). A successful display of the CotB-VP8* fusion protein on the spore surface was confirmed by Western blot and immunofluorescence microscopy analysis. The capacity for immune response generated after immunization with the recombinant strain was evaluated in a mouse model. The intestinal fecal IgA and serum IgG were detected by enzyme-linked-immunosorbent serologic assay (ELISA). Importantly, recombinant strain spores could elicit strong specific mucosal and humoral immune responses. These encouraging results suggest that recombinant B. subtilis BV could provide a strategy for a potential novel application approach to the development of a new and safe mucosal subunit vaccine against porcine rotavirus. View Full-Tex
%U https://www.mdpi.com/1420-3049/24/20/3793