%0 Journal Article
%T The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium
%A Filip Thieme
%A Hana Kubatova
%A Ivana Kralova Lesna
%A Jiri Fronek
%A Libor Janousek
%A Rudolf Poledne
%A Sona Cejkova
%J Cell Adhesion & Migration
%D 2019
%R https://doi.org/10.1080/19336918.2019.1644856
%X ABSTRACT Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro£¿inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1¦Â,TNF-¦Á,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1¦Â and TNF-¦Á in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1¦Â, TNF-¦Á, MCP-1, IL-10, and RANTES, with IL-1¦Â and TNF£¿¦Á having the strongest impact
%U https://www.tandfonline.com/doi/full/10.1080/19336918.2019.1644856