%0 Journal Article
%T Impact of frequency of denture cleaning on microbial and clinical parameters ¨C a bench to chairside approach
%A Andrew MacInnes
%A Anto Jose
%A Clare Brown
%A David Bradshaw
%A Debbie McKenzie
%A Douglas Robertson
%A Gordon Ramage
%A Jeremy Bagg
%A Laura Cross
%A Leighann Sherry
%A Lindsay O¡¯Donnell
%A Marta Czesnikiewicz-Guzik
%A Paola Gomez Pereira
%A Roshan Varghese
%A Shauna Culshaw
%A Susmita Sanyal
%J Journal of Oral Microbiology
%D 2019
%R https://doi.org/10.1080/20002297.2018.1538437
%X ABSTRACT Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident£¿ denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega£¿ denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. Results: The in vitro model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both in vitro and in vivo studies showed that denture plaque biofilm composition were affected by different treatment arms. Conclusions: This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Clinicaltrials.gov registration: NCT02780661
%U https://www.tandfonline.com/doi/full/10.1080/20002297.2018.1538437