%0 Journal Article
%T GRIM-19 over-expression represses the proliferation and invasion of orthotopically implanted hepatocarcinoma tumors associated with downregulation of Stat3 signaling.
%A Chen JY
%A Cheng HJ
%A Du YW
%A He P
%A Huang D
%A Kong DX
%A Lin Y
%A Meng XW
%A Sun X
%A Wu S
%A Yang LL
%A Zhao LJ
%J BioScienceTrends
%D 2019
%R BioScience Trends. 2019 ;13(4):342-350. (DOI: 10.5582/bst.2019.01185)
%X SUMMARY: The retinoid-interferon-induced mortality-19 (GRIM-19) gene has been identified as a negative regulator associated with tumor development. The current study created a model of an orthotopically implanted hepatocarcinoma tumor to verify the inhibitory effect of GRIM- 19 in vivo. After treatment with GRIM-19 carried by attenuated Salmonella, transplanted tumors were measured with an Imaging System. The expression of GRIM-19, Stat3/ p-Stat3, cyclinD1, CDK4, PCNA, Bax/Bcl-2, cleaved caspase-9/3, VEGF, and MMP-2/9 was determined using immunohistochemistry and Western blot analysis. The cell cycle was assessed using flow cytometry (FCM). Apoptosis was determined using FCM and a TUNEL assay. Results indicated that GRIM-19 overexpression resulted in inhibition of peritoneal metastasis, induction of cell cycle arrest, and apoptosis in vivo. In addition, the expression of Stat3/p-Stat3 was down-regulated by GRIM-19. These results suggest that GRIM-19 overexpression could suppress the growth of orthotopically implanted hepatocarcinoma tumors by reversing the regulation of the Stat3 signaling pathway. This approach could potentially be a powerful treatment for hepatocarcinoma. Key Words: GRIM-19, hepatocellular carcinoma, orthotopically implanted tumor, Stat3, apoptosis Full Text: PDF(6956KB
%U http://www.biosciencetrends.com/getabstract.php?id=1760