%0 Journal Article
%T 百脉根Rboh基因家族在共生固氮中的功能研究
Function of Rboh gene family in Lotus japonicas during symbiotic nitrogen fixation
%A 凌颖
%A 张婷婷
%A 端木德强
%A 张忠明
%A 曹扬荣
%J 华中农业大学学报
%D 2019
%X 为探索植物呼吸爆发氧化酶（respiratory burst oxidase homologue,Rboh）基因家族在共生固氮中的功能和作用机制，利用分子生物学、细胞生物学和遗传学等方法，围绕百脉根Rboh基因家族中在根瘤中特异性表达的基因LjRbohA（Lj5g3v1497840）以及在根瘤中高表达的基因LjRbohB（Lj6g3v1549190）和LjRbohC（Lj5g3v1497820），分别对其蛋白的亚细胞定位、基因的表达模式、基因的启动子活性及蛋白的功能进行了研究。结果显示：LjRbohA、LjRbohB和LjRbohC蛋白都定位在植物的细胞膜上；LjRbohA、LjRbohB和LjRbohC基因在接种根瘤菌后表达上调；LjRobhA和LjRobhB基因在根瘤、维管束和侧根原基表达；利用CRISPR Cas9技术对LjRbohA、LjRbohB和LjRbohC 3个基因分别进行敲除导致结瘤数目减少。这些研究结果表明，LjRbohA、LjRbohB和LjRbohC这 3个基因可能都参与调控百脉根与根瘤菌的共生结瘤过程。
genes including RbohA(Lj5g3v1497840),RbohB(Lj6g3v1549190) and RbohC (Lj5g3v1497820) were found to be primarily expressed in nodules based on the transcriptions of Rboh genes in symbiosis in Lotus japonicus. Their function involved in symbiosis were further studied in Lotus japonicus.The subcellular location of proteins,the expression pattern of Rboh genes,the proteins function and the promoter activity of Rboh genes in Lotus japonicas were studied with methods of molecular biology,cell biology and genetics. The results showed that LjRbohA,LjRbohB and LjRbohC proteins were localized at the plasma membrane.The transcript of LjRbohA,LjRbohB and LjRbohC accumulated abundantly in roots and nodules. The activity of LjRbohA and LjRbohB promoters were detected at vascular tissue,nodule,and the lateral root primordia.The numbers of nodules were reduced in LjRbohB,LjRbohA LjRbohC and LjRbohA LjRbohB LjRbohC knockout transgenic roots compared with control transgenic roots. It is indicated that these three Rbohs might have an overlapping function in regulating nitrogen fixation symbiosis in L. japonicus.
%K 百脉根 共生固氮 植物呼吸爆发氧化酶 活性氧 根瘤
Lotus japonicus symbiotic nitrogen fixation respiratory burst oxidase homologue（Rboh） reactive oxygen species(ROS) nodule
%U http://hnxbl.cnjournals.net/hznydxzr/ch/reader/view_abstract.aspx?file_no=20190101&flag=1