%0 Journal Article
%T 大豆快生根瘤菌SfHH103_03182基因突变体的构建和共生固氮表型分析<br>Mutant construction and symbiotic nitrogen fixation phenotype analysis of soybean rhizobia SfHH 103_03182
%A 杜思
%A 曾小波
%A 李友国
%J 华中农业大学学报
%D 2019
%X 以大豆快生根瘤菌HH103中glnK同源基因SfHH103_03182为研究对象，采用pK19mob单插入失活技术构建该基因的插入失活突变体，菌落PCR和测序分析表明突变体构建正确。植物盆栽试验结果显示，与接种野生型菌株HH103相比，突变体接种植株的根瘤数量增多，植株鲜质量和根瘤鲜质量增加，根瘤固氮酶活无显著差异。在不同浓度氮素处理和盆栽条件下，对共生表型的检测表明突变体接种植物的根瘤数量没有显著变化。<br>The GlnK protein is a PⅡ family protein existing widely in bacteria,archaea and plants and involved in regulating nitrogen metabolism. The insertion mutant of glnK homologue gene SfHH103_03182 in Sinorhizobium fredii HH103 was constructed. The results of PCR and sequence analyses confirmed that the mutant was correctly constructed. The results of pot plant experiments showed that the nodule number of the plant inoculated with the mutant,the fresh weight of plants and the fresh weight of root nodules increased compared with inoculated with wild type strain HH103. There was no significant difference in root nodule nitrogenase activity. The results of symbiotic phenotype tested on host plant soybean at different concentrations of nitrogen levels showed that there was no significant changes in the number of root nodules in the mutant inoculated plants.
%K 大豆快生根瘤菌HH103 PⅡ蛋白 插入突变株 共生固氮 高氮处理<br>Sinorhizobium fredii HH103 PⅡ protein inserted mutant symbiotic nitrogen fixation high nitrogen stress
%U http://hnxbl.cnjournals.net/hznydxzr/ch/reader/view_abstract.aspx?file_no=20190106&flag=1