%0 Journal Article
%T The human somatostatin receptor type 2 as an imaging and suicide reporter gene for pluripotent stem cell-derived therapy of myocardial infarction
%A Bryan Holvoet
%A Catherine M. Verfaillie
%A Christophe M. Deroose
%A Esther Wolfs
%A Hubert Vanbilloen
%A Ivo Lambrichts
%A Katrien Neyrinck
%A Maurilio Sampaolesi
%A Natacha Breuls
%A Olivier Gheysens
%A Robin Duelen
%A Uwe Himmelreich
%A Willy Gsell
%A Wouter Oosterlinck
%J Theranostics
%D 2018
%I Ivyspring International Publisher
%R 10.7150/thno.22980
%X Rationale: Pluripotent stem cells (PSCs) are being investigated as a cell source for regenerative medicine since they provide an infinitive pool of cells that are able to differentiate towards every cell type of the body. One possible therapeutic application involves the use of these cells to treat myocardial infarction (MI), a condition where billions of cardiomyocytes (CMs) are lost. Although several protocols have been developed to differentiate PSCs towards CMs, none of these provide a completely pure population, thereby still posing a risk for neoplastic teratoma formation. Therefore, we developed a strategy to (i) monitor cell behavior noninvasively via site-specific integration of firefly luciferase (Fluc) and the human positron emission tomography (PET) imaging reporter genes, sodium iodide symporter (hNIS) and somatostatin receptor type 2 (hSSTr2), and (ii) perform hSSTr2-mediated suicide gene therapy via the clinically used radiopharmacon 177Lu-DOTATATE.
%K noninvasive imaging
%K stem cell therapy
%K myocardial infarction
%K suicide gene
%U http://www.thno.org/v08p2799.htm