%0 Journal Article
%T Diagnostic targETEd seQuencing adjudicaTion (DETEQT): Algorithms for Adjudicating Targeted Infectious Disease Next-Generation Sequencing Panels
%A Adrienne T. Hall
%A Amanda S. Graham
%A Chien-Chi Lo
%A Christina E. Douglas
%A Christopher P. Stefan
%A Jeffrey W. Koehler
%A Patrick S. Chain
%A Timothy D. Minogue
%A Turner A. Conrad
%J The Journal of Molecular Diagnostics
%D 2019
%R 10.1016/j.jmoldx.2018.08.008
%X Next-generation sequencing (NGS) for infectious disease diagnostics is a relatively
new and underdeveloped concept. If this technology is to become a regulatory-grade
clinical diagnostic, standardization in the form of locked-down assays and firmly
established underlying processes is necessary. Targeted sequencing, specifically by
amplification of genomic signatures, has the potential to bridge the gap between PCR-
and NGS-based diagnostics; however, existing NGS assay panels lack validated analytical
techniques to adjudicate high background and error-prone NGS data.
%U https://jmd.amjpathol.org/article/S1525-1578(18)30185-5/fulltext