%0 Journal Article
%T High Level Estradiol Induces EBV Reactivation and EBV gp350/220(+)CD138(+)
Double-positive B Cell Population in GravesĄŻ Disease Patients and Healthy
Controls
%A Katsumi Higaki
%A Kazuhiko Hayashi
%A Keiko Nagata
%A Masako Kato
%A Michiko Matsushita
%A Satoshi Kuwamoto
%A Sayuri Hara
%A Yuji Nakayama
%J Archive of "Yonago Acta Medica".
%D 2019
%R 10.33160/yam.2019.06.010
%X GravesĄŻ disease occurs predominantly in women. Epstein-Barr virus (EBV) mainly persists in human B lymphocytes, and its reactivation stimulates antibody production. We previously suggested that the EBV reactivation-induced production of TRAb and IgM at 100 nM estradiol (pregnant level) was lower than that at 0 nM estradiol and that class switch recombination may be increased by estradiol. In this study, we examined the effect of estradiol on EBV reactivation. We identified the expression of EBV-glycoprotein 350/220 (gp350/220) in the late phase of reactivation and plasma cell differentiation of EBV-infected cells using 72A1 antibody and CD138 antibody, respectively. We found the mean ratio of gp 350/220(+) CD138(+) cells at 100 nM estradiol was higher than that at 0 nM estradiol. These results suggested that EBV-infected cells could survive with keeping the ability of antibody production in 100 nM estradiol, which is consistent with the improvement of GravesĄŻ disease during maternity and exacerbation postpartum
%K anti-gp350/220 antibody (72A1)
%K Epstein-Barr virus
%K estradiol
%K GravesĄŻ disease
%K reactivation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584263/