%0 Journal Article
%T Understanding the Role of Fatty Acid Substrates on Primycin Biosynthesis by Saccharomonospora azurea During Batch Fermentation
%A Andrea Kov¨¢cs-Valasek
%A D¨¦nes Seffer
%A G¨¢bor Dr¨¢vav£¿lgyi
%A Ildik¨® Kerepesi
%A J¨®zsef P¨¦ter Pallos
%A M¨¢ria Seffern¨¦ Szalai
%A M¨¢rk Kov¨¢cs
%J Natural Product Communications
%@ 1555-9475
%D 2019
%R 10.1177/1934578X19858210
%X Primycin is a 36-membered marginolactone antibiotic that is biosynthesized through the modular type I polyketide synthase pathway produced by Saccharomonospora azurea, a Gram-positive, soil-dwelling filamentous bacteria. In industrial-scale batch fermentation the primycin-producing strain is cultivated in a complex fermentation media empirically optimized for antibiotic production. To determine the role of various fatty acids on primycin production, the effect of stearic acid (C18:0), palmitic acid (C16:0), lauric acid (C12:0), capric acid (C10:0), enanthic acid (C7:0), caproic acid (C6:0), and butyric acid (C4:0) in growth medium was studied. Our results clearly show that palmitic acid was a better alternative of the originally applied stearic acid in all tested concentrations, while 4.5 g/L proved to be the most effective
%K Saccharomonospora azurea
%K palmitic acid
%K polyketide synthase
%K primycin
%K batch fermentation
%U https://journals.sagepub.com/doi/full/10.1177/1934578X19858210