%0 Journal Article
%T Monitoring of impaired phagocytic function of Kupffer cells in an obstructive cholangitis rat model using superparamagnetic iron oxide MRI and contrast
%A Jae Hwan Lee
%A Joon Koo Han
%A Jung Hoon Kim
%A Seunghyun Lee
%J Acta Radiologica
%@ 1600-0455
%D 2019
%R 10.1177/0284185118784978
%X Kupffer cells (KC) have an important role in the host defense in obstructive cholangitis. Non-invasive monitoring of phagocytic function of KC is pivotal. Several studies showed the possibility of non-invasive monitoring of phagocytic function of KC using superparamagnetic iron oxide-enhanced magnetic resonance imaging (SPIO-MRI) or contrast-enhanced ultrasound (CEUS). To investigate the serial change of KC function using SPIO-MRI and CEUS and whether the SPIO-MRI parameter correlates with the CEUS parameter in obstructive cholangitis rat models. With our institutional Animal Care and Use Committee approval, 19 rats (common bile duct ligation [CBDL]: n£¿=£¿9; control: n£¿=£¿10) underwent SPIO-MRI and CEUS at baseline, two, and four weeks after CBDL. The relative signal loss (RSL) of T2* value on SPIO-MRI and Kupffer phase parenchymal echogenicity (KPE) on CEUS were measured. The correlation between SPIO-MRI and CEUS parameters were compared with KC count. In CBDL group, RSL and KPE had significantly decreased (72.1% to 29.5%, 2.7 to 0.4) at four weeks compared with those in the control group (68.2% to 58.3%, 2.5 to 3.0, P£¿<£¿0.05). During the follow-up period, RSL showed significantly positive correlations with KPE (P£¿=£¿0.007). In addition, at four weeks, we found RSL was positively correlated with KPE (¦Ñ£¿=£¿0.750, P£¿=£¿0.002). KC count was negatively correlated to RSL and KPE at four weeks (¦Ñ£¿=£¿£¿0.771, P£¿=£¿0.001 and ¦Ñ£¿=£¿£¿0.644, P£¿=£¿0.013). SPIO-MRI and CEUS may be equally useful for monitoring the serial changes of KC phagocytic function in vivo
%K Contrast-enhanced ultrasound
%K superparamagnetic iron oxide-enhanced MRI
%K Kupffer cells
%K obstructive cholangitis
%K animal study
%U https://journals.sagepub.com/doi/full/10.1177/0284185118784978