%0 Journal Article
%T Mouse Model of Tracheal Replacement With Electrospun Nanofiber Scaffolds
%A Cameron A. Best
%A Christopher K. Breuer
%A Jed Johnson
%A Michaela Henderson
%A Nakesha King
%A Sayali Dharmadhikari
%A Tendy Chiang
%J Annals of Otology, Rhinology & Laryngology
%@ 1943-572X
%D 2019
%R 10.1177/0003489419826134
%X The clinical experience with tissue-engineered tracheal grafts (TETGs) has been fraught with graft stenosis and delayed epithelialization. A mouse model of orthotopic replacement that recapitulates the clinical findings would facilitate the study of the cellular and molecular mechanisms underlying graft stenosis. Electrospun nanofiber tracheal scaffolds were created using nonresorbable (polyethylene terephthalate + polyurethane) and co-electrospun resorbable (polylactide-co-caprolactone/polyglycolic acid) polymers (n = 10/group). Biomechanical testing was performed to compare load displacement of nanofiber scaffolds to native mouse tracheas. Mice underwent orthotopic tracheal replacement with syngeneic grafts (n = 5) and nonresorbable (n = 10) and resorbable (n = 10) scaffolds. Tissue at the anastomosis was evaluated using hematoxylin and eosin (H&E), K5+ basal cells were evaluated with the help of immunofluorescence testing, and cellular infiltration of the scaffold was quantified. Micro computed tomography was performed to assess graft patency and correlate radiographic and histologic findings with respiratory symptoms. Synthetic scaffolds were supraphysiologic in compression tests compared to native mouse trachea (P < .0001). Nonresorbable scaffolds were stiffer than resorbable scaffolds (P = .0004). Eighty percent of syngeneic recipients survived to the study endpoint of 60 days postoperatively. Mean survival with nonresorbable scaffolds was 11.40 ¡À 7.31 days and 6.70 ¡À 3.95 days with resorbable scaffolds (P = .095). Stenosis manifested with tissue overgrowth in nonresorbable scaffolds and malacia in resorbable scaffolds. Quantification of scaffold cellular infiltration correlated with length of survival in resorbable scaffolds (R2 = 0.95, P = .0051). Micro computed tomography demonstrated the development of graft stenosis at the distal anastomosis on day 5 and progressed until euthanasia was performed on day 11. Graft stenosis seen in orthotopic tracheal replacement with synthetic tracheal scaffolds can be modeled in mice. The wide array of lineage tracing and transgenic mouse models available will permit future investigation of the cellular and molecular mechanisms underlying TETG stenosis
%K tissue engineered trachea
%K tracheal stenosis
%K biomaterials
%K micro computed tomography
%U https://journals.sagepub.com/doi/full/10.1177/0003489419826134