%0 Journal Article
%T 2,3,7,8
%A Jia Cao
%A Jie Xu
%A Liyun Gao
%A Tao Wang
%A Weidong Wu
%A Xiao Li
%J Dose
%@ 1559-3258
%D 2019
%R 10.1177/1559325818786822
%X 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a well-known environmental teratogenic effector for cleft palate. Transforming growth factor 3 (TGF-¦Â3) is an essential growth factor for palatogenesis. The objective of this study is to clarify the effects of TCDD and TGF-¦Â3 in mouse embryonic palatal mesenchymal (MEPM) cells. The effects of 10 nM TCDD, 10 ng/mL TGF-¦Â3, or a combination of 10 nM TCDD and 10 ng/mL TGF-¦Â3 on MEPM cells were revealed by cell and biological methods. With the increase in TCDD (0.5-10 nM), the expression of TGF-¦Â3 increased, but at TCDD concentrations greater than 10 nM, the expression of TGF-¦Â3 reduced. The viabilities of MEPM cells decreased in the 10 nM TCDD-treated group. But the viabilities increased in the 10 ng/mL TGF-¦Â3-treated group, and the viabilities were intermediate in the group treated with a combination of 10 nM TCDD and 10 ng/mL TGF-¦Â3. This phenomenon was the same as that of the motilities. In addition, we found that the expression of p-Smad2, p-Smad3,and Smad7 were increased by TCDD, TGF-¦Â3, combination of TCDD and TGF-¦Â3, but the expression of Smad4 were decreased by TCDD, TGF-¦Â3, combination of TCDD and TGF-¦Â3. These data revealed that TCDD and TGF-¦Â3 interacted and affected MEPM cells
%K TCDD
%K MEPM cells
%K TGF-¦Â3
%K TGF-/Smad signaling
%U https://journals.sagepub.com/doi/full/10.1177/1559325818786822