%0 Journal Article
%T Thermomechanical and in vitro biological characterization of injection
%A Carlos Rodrigo de Mello Roesler
%A Gean Vitor Salmoria
%A Izabelle de Mello Gindri
%A Liliane Pimenta de Melo
%A Nicola Contessi Negrini
%A Silvia Far¨¨
%J Journal of Applied Biomaterials & Functional Materials
%@ 2280-8000
%D 2019
%R 10.1177/2280800019831599
%X To evaluate the thermomechanical and in vitro biological response of poly(lactic-co-glycolic acid) (PLGA) plates for craniofacial reconstructive surgery. PLGA 85/15 craniofacial plates were produced by injection molding by testing two different temperatures (i.e., 240ˇăC, PLGA_lowT, and 280ˇăC, PLGA_highT). The mechanical properties of the produced plates were characterized by three-point bending tests, dynamic mechanical analysis, and residual stress. Crystallinity and thermal transitions were investigated by differential scanning calorimetry. Finally, in vitro cell interaction was evaluated by using SAOS-2 as cell model. Indirect cytotoxicity tests (ISO 10-993) were performed to prove the absence of cytotoxic release. Cells were then directly seeded on the plates and their viability, morphology, and functionality (ALP) checked up to 21 days of culture. A similar performance of PLGA_lowT and PLGA_highT plates was verified in the three-point bending test and dynamic mechanical analyses. Also, the two processing temperatures did not influence the in vitro cell interaction. Cytotoxicity and ALP activity were similar for the PLGA plates and control. Cell results demonstrated that the PLGA plates supported cell attachment and proliferation. Furthermore, energy-dispersive X-ray spectroscopy revealed the presence of sub-micron particles, which were identified as inorganic mineral deposits resulting from osteoblast activity. The present work demonstrated that the selected processing temperatures did not affect the material performance. PLGA plates showed good mechanical properties for application in craniofacial reconstructive surgery and adequate biological properties
%K Injection molding
%K PLGA
%K craniofacial plate
%K thermomechanical properties
%K in vitro biological tests
%U https://journals.sagepub.com/doi/full/10.1177/2280800019831599