%0 Journal Article
%T Sensitive detection of porcine circovirus 3 by droplet digital PCR
%A Hecheng Meng
%A Lei Shi
%A Lili Li
%A Yuqi Liu
%J Journal of Veterinary Diagnostic Investigation
%@ 1943-4936
%D 2019
%R 10.1177/1040638719847686
%X Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/¦ĚL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection
%K detection
%K droplet digital PCR
%K porcine circovirus 3
%U https://journals.sagepub.com/doi/full/10.1177/1040638719847686