%0 Journal Article
%T Nickel
%A Bing Xia
%A Chang Li
%A Rui Xin
%A Runan Qin
%A Shengyuan Wang
%A Yonghui Wu
%A Yue Wang
%J Toxicology and Industrial Health
%@ 1477-0393
%D 2019
%R 10.1177/0748233719828589
%X Nickel (Ni) is a metal known to be a human carcinogen that occupational workers can be exposed to during the process of Ni refining. We investigated the molecular mechanism of inflammation that is induced by Ni-refining dust in a factory, using concentrations of 0, 25, 50, and 100 米g/mL for 24 h and 48 h, in vitro. Quantitative real-time polymerase chain reactions (qRT-PCR), Western blot analysis, and enzyme-linked immunosorbent assays (ELISA) were used to detect the transcriptional expression levels of nuclear factor-百B (NF-百B), tumor necrosis factor-汐 (TNF-汐), interleukin-1汕 (IL-1汕), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Results showed that Ni-refining dust decreased the secretion of IL-6 under the experimental conditions. In contrast, Ni-refining dust activated NF-百B expression and stimulated the secretion of TNF-汐, IL-1汕, iNOS, and COX-2 in a dose- and time-dependent manner. To summarize, we demonstrated that exposure to Ni-refining dust can induce the expression of NF-百B in NIH/3T3 cells and the secretion of inflammation related factors. This provides a new basis for further study of the inflammatory effects of Ni-refining dust
%K Ni-refining dust
%K cytotoxicity
%K nuclear factor-百B
%K cytokines
%K inflammation
%U https://journals.sagepub.com/doi/full/10.1177/0748233719828589