%0 Journal Article
%T Antagonistic effect of co
%A Athena Rafieepour
%A Fariba Khodagholi
%A Habibollah Peirovi
%A Jalal Pourahmad
%A Mansour Rezazadeh Azari
%A Meisam Omidi
%A Yadollah Mehrabi
%A Yousef Mohammadian
%J Toxicology and Industrial Health
%@ 1477-0393
%D 2019
%R 10.1177/0748233719854570
%X In theenvironment, co-exposure to short-multiwalled carbon nanotubes (S-MWCNTs) and polycyclic aromatic compounds (PAHs) has been reported. In the co-exposure condition, the adsorption of PAHs onto MWCNTs may reduce PAHs toxic effect. The objective of this study was to investigate the cytotoxicity of S-MWCNTs and benzo[a]pyrene (B[a]P) individually, and in combination in human lung cell lines (A549). The adsorption of B[a]P onto MWCNTs was measured spectrometrically. In vitro toxicity was assessed through cell viability, reactive oxygen species (ROS) generation, apoptosis, and 8-hydroxy-2กไ-deoxyguanosine (8-OHdG) generation experiments. The S-MWCNTs demonstrated cytotoxicity through the generation of ROS, apoptosis, and 8-OHdG in A549 cells. Co-exposure to S-MWCNTs and B[a]P demonstrated a significant reduction in ROS generation and apoptosis compared with the sum of their separate toxic effects at the same concentrations. Decreasing the bioavailability of B[a]P by MWCNT interaction is the probable reason for the antagonistic effects of the co-exposure condition. The findings of this study will contribute to a better understanding of the health effects of co-exposures to air pollutants and could be a starting point for modifying future health risk assessments
%K Short-multiwalled carbon nanotubes
%K benzo[a]pyrene
%K co-exposure
%K antagonistic effect
%K lung cells
%U https://journals.sagepub.com/doi/full/10.1177/0748233719854570