%0 Journal Article
%T Development of an Efficient Analytical Method for the Extraction and Analysis of Biocide Contents from the Textile Test Specimens on LC-DAD
%A Jahangir
%A Muhammad
%A Khan
%A Uzman
%J -
%D 2020
%R https://doi.org/10.1155/2020/3047961
%X Biocides are frequently used in the manufacturing of textiles that are in direct contact with human skin. Recently regulated biocides do not have validated methods for testing; so, their presence cannot be estimated in the consumer products. Hence a rapid method was developed for the separation and quantitative analysis of biocide contents (2-methyl-4-isothaizolin (MIT), 5-chloro-2-methyl-4-isothaizolin-3-one (CIT), 2-octo-4-isothaizolin-3-one (OIT), and 5-chloro-2-(2,4-dichlorophenxy) phenol (triclosan)) from the textile test specimens. Test specimens were extracted with methanolic sonication and purified by centrifugation and filtration. Biocide contents were separated at C18 column with 0.4% acetic acid: methanol (1£¿:£¿1 v/v) under isocratic mode and detected at 280£¿nm wavelength. Pretreatment factors such as extraction solvent, extraction method, dilution ratio, and extraction time were optimized initially and plotted calibration curve showed regression (r2£¿¡Ý£¿0.9995) in the range of 1.0¨C5.0£¿mg£¿L£¿1. Recoveries were between 95% and 108% with the relative standard deviation£¿¡Ü£¿4%. Limits of detection (LODs) were between 0.06£¿mg£¿L£¿1 and 0.12£¿mg£¿L£¿1 and limits of quantification (LOQs) were between 0.21£¿mg£¿L£¿1 and 0.38£¿mg£¿L£¿1. From the results, conclusion was made that the method can achieve the purpose of quantitative detection and the analysis of real test specimens verified the reliability of this method
%U https://www.hindawi.com/journals/jamc/2020/3047961/