%0 Journal Article
%T A new human adipocyte model with PTEN haploinsufficiency
%A Anna Kirstein
%A Antje Berthold
%A Antje Garten
%A Antje K£¿rner
%A Astrid Tannert
%A Franziska K£¿ssner
%A Gordian L. Schmid
%A Kathrin L
%A Kiess
%A Martin Wabitsch
%A Michael Schaefer
%A Norman H£¿ndel
%A Wiel
%A graf
%J Adipocyte
%D 2020
%R https://doi.org/10.1080/21623945.2020.1785083
%X ABSTRACT Few human cell strains are suitable and readily available as in vitro adipocyte models. We used resected lipoma tissue from a patient with germline phosphatase and tensin homolog (PTEN) haploinsufficiency to establish a preadipocyte cell strain termed LipPD1 and aimed to characterize cellular functions and signalling pathway alterations in comparison to the established adipocyte model Simpson-Golabi-Behmel-Syndrome (SGBS) and to primary stromal-vascular fraction cells. We found that both cellular life span and the capacity for adipocyte differentiation as well as adipocyte-specific functions were preserved in LipPD1 and comparable to SGBS adipocytes. Basal and growth factor-stimulated activation of the PI3 K/AKT signalling pathway was increased in LipPD1 preadipocytes, corresponding to reduced PTEN levels in comparison to SGBS cells. Altogether, LipPD1 cells are a novel primary cell model with a defined genetic lesion suitable for the study of adipocyte biology
%U https://www.tandfonline.com/doi/full/10.1080/21623945.2020.1785083