%0 Journal Article
%T The effect of Acot2 overexpression or downregulation on the preadipocyte differentiation in Chinese Red Steppe cattle
%A Cheng Xiao
%A Guoliang Zhang
%A Jiajia Xue
%A Jian Wu
%A Lichun Zhang
%A Lihong Qin
%A Lixiang Liu
%A Xiaotong Luo
%A Yang Cao
%A Yang Lv
%A Yanli Wang
%A Yi Gao
%A Zhongchang Hu
%J Adipocyte
%D 2020
%R https://doi.org/10.1080/21623945.2020.1776553
%X ABSTRACT The quality and nutritional value of beef is closely linked to its content of intramuscular fat (IMF). The differentiation of preadipocytes and the deposition of lipid droplets in the adipocytes are the key to regulate the IMF content. The differentiation of adipocytes is regulated by a series of transcription factors and genes. Acyl-CoA thioesterase 2 (Acot2) hydrolyzes the acyl-coenzyme A (CoA) into free fatty acids and CoA and has the potential to maintain the free fatty acids and acyl CoA at the cellular level. In this work, we detected the expression of the Acot2 gene during the adipocyte differentiation in Chinese Red Steppe cattle, and then interfered and overexpressed the Acot2 gene in the preadipocytes to explore its regulatory role in the adipocyte differentiation. The results showed that the expression and regulation of Acot2 mainly occurred at the later stage of the adipocyte differentiation. The interference with the Acot2 gene significantly inhibited the lipid droplets accumulation and triglyceride content, while its overexpression significantly promoted both of them. The results of this study show that the Acot2 gene is a positive regulator of the adipocyte differentiation and may become a new target to improve the beef quality
%U https://www.tandfonline.com/doi/full/10.1080/21623945.2020.1776553