%0 Journal Article
%T MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9
%A Guihua Li
%A Jianhui Xu
%A Wanhong Zhu
%A Zhancheng Ma
%A Zhihua Liang
%J Bioengineered
%D 2020
%R https://doi.org/10.1080/21655979.2019.1706287
%X ABSTRACT Non-small-cell lung cancer (NSCLC) is the main pathological type of lung cancer and has a low overall five-year survival rate. miR-187 has been reported to play major roles in various tumor types. In this study, we explored the impact of miR-187 on NSCLC. qRT-PCR results demonstrated that miR-187 expression is lower in NSCLC and cancer cells than normal tissues and normal lung cells. miR-187 expression levels are associated with tumor size, TNM stage and overall survival rate. MTS and colony formation assays showed that high miR-187 expression inhibits NSCLC cell proliferation and colony formation ability, and flow cytometry showed that miR-187 overexpression induces cell cycle arrest at the G0/G1 phase. A luciferase reporter assay showed that FGF9 is a target of miR-187. miR-187 overexpression reduces the expression of FGF9, cyclin D1 CDK4 and CDK6. Therefore, miR-187 may present a new NSCLC treatment target by regulates cyclins-related protein expression. Graphical abstrac
%U https://www.tandfonline.com/doi/full/10.1080/21655979.2019.1706287