%0 Journal Article
%T Unspecific DNA recombination in AdipoqCre-ERT2 ¨C mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent
%A Andreas Lindhorst
%A Ingo Bechmann
%A Martin Gericke
%J Adipocyte
%D 2020
%R https://doi.org/10.1080/21623945.2019.1701394
%X ABSTRACT Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ERT2). We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ERT2), which were crossed to a tdTomato reporter mouse to visualize the site of recombination on a single-cell resolution. Albeit tamoxifen induced tdTomato expression in this model, also non-stimulated background recombination (¡®Cre leakage¡¯) was detected in AT of untreated Adipoq-CreERT2xTDTO mice in vivo. Quantification of Cre leakage revealed age, sex and genotype as factors impacting on non-induced Cre recombination
%U https://www.tandfonline.com/doi/full/10.1080/21623945.2019.1701394