%0 Journal Article
%T Characterization of LcGAPC and its transcriptional response to salt and alkali stress in two ecotypes of Leymus chinensis (Trin.) Tzvelev
%A Fanghui Yang
%A Jizhe Cui
%A Qian Lu
%A Xin Liu
%A Xue Meng
%J Biotechnology & Biotechnological Equipment
%D 2020
%R https://doi.org/10.1080/13102818.2020.1719020
%X Abstract Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a housekeeping protein which plays various roles in non-metabolic processes in addition to its role in glucose catabolism. There are several GAPDH isoforms in plant cells. In this study, we cloned the full-length cDNA encoding the isoform GAPC in two ecotypes of Leymus chinensis (Trin.) Tzvelev, gray¨Cgreen type and yellow¨Cgreen type. The LcGAPC sequence includes an open reading frame (ORF) of 1014£¿bp encoding 337 amino acids. The predicted molecular weight is 36.5£¿kDa, and the pI is 6.19. The mRNA expression of LcGAPC decreased at 24£¿h and then increased significantly after 7 d after 400£¿mmol/L NaCl stress. At 200£¿mmol/L mixed alkali stress (NaHCO3:Na2CO3=9:1), the LcGAPC expression level gradually increased as time increased in the two ecotypes of L. chinensis. The results suggested that LcGAPC is a stress-inducible gene that might play a role in the salt and alkali stress response. This study provided a basis to further study the mechanism of expression characteristics under salt and alkali stress conditions of L. chinensis
%U https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1719020