%0 Journal Article
%T "First Detection of PER-Type Extended-Spectrum ¦Â-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "
%J -
%D 2019
%X Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum ¦Â-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the ¦Â-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso
%K Broad-spectrum Beta-lactamases (ESBL)
%K PER Gene
%K PCR
%U http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=246&doi=10.11648/j.ijbbmb.20180401.12