%0 Journal Article
%T Oligopeptide-side chained alginate nanocarrier for melittin-targeted chemotherapy
%J -
%D 2019
%R https://doi.org/10.1038/s41428-019-0191-6
%X A melittin-targeting drug carrier was successfully synthesized by the grafting of sodium alginate to an oligopeptide via an amidation method at different oligopeptide:alginate unit molar ratios. The average sizes of the oligopeptide¨Calginate nanoparticles formed in the presence of 1£¿mM CaCl2 decreased with increasing oligopeptide contents, indicating intramolecular interactions between oligopeptide-side chains. While the doxorubicin-loading efficiency on nanoparticles (0.1:1) was similar to that of alginate nanoparticles, the melittin-loading onto oligopeptide¨Calginate nanoparticles (0.1:1) was more than double that onto alginate nanoparticles, suggesting the specific interaction of melittin with the oligopeptide-side chain in the oligopeptide¨Calginate nanoparticles. While 2.5£¿¦ÌM free melittin caused almost no damage to Caco-2 cells, more than 80% of cells did not survive under the dose of 2.5£¿¦ÌM melittin-loaded oligopeptide¨Calginate nanoparticles. The results confirm that the derivation of an oligopeptide-side chain in alginate offers a specific binding site for melittin and effectively works in cancer chemotherapy
%U https://www.nature.com/articles/s41428-019-0191-6