%0 Journal Article
%T Approaches for Studying Ribosome Specialization
%J -
%D 2019
%R https://doi.org/10.1016/j.tibs.2019.01.008
%X Graphical Abstract View Large Image Figure Viewer Download Hi-res image Contrary to the textbook model, recent measurements demonstrated unexpected diversity in ribosomal composition that likely enables specialized translational functions. Methods based on liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) enable direct quantification of ribosomal proteins with high specificity, accuracy, and throughput. LC-MS/MS can be ¡®top-down¡¯, analyzing intact proteins, or more commonly ¡®bottom-up¡¯, where proteins are digested to peptides prior to analysis. Changes to rRNA can be examined using either LC-MS/MS or sequencing-based approaches. The regulation of protein synthesis by specialized ribosomes can be examined by multiple methods. These include the popular ¡®Ribo-Seq¡¯ method for analyzing ribosome density on a given mRNA, as well as LC-MS/MS approaches incorporating pulse-labelling with stable isotopes (SILAC) to monitor protein synthesis and degradation
%U https://www.cell.com/trends/biochemical-sciences/fulltext/S0968-0004(19)30017-9