%0 Journal Article
%T Interrogating Precision Electrophile Signaling
%J -
%D 2019
%R https://doi.org/10.1016/j.tibs.2019.01.006
%X Graphical Abstract View Large Image Figure Viewer Download Hi-res image Understanding the targets and signaling roles of reactive electrophilic species (RES) at a specific cellular space and time has long been hampered by the reliance of the field on the bulk administration of excess RES from outside of cells and/or animals. Uncontrolled bolus methods provide limited understanding of target engagement for these individual nonenzymatic RES-modification events. REX technologies [targetable reactive electrophiles and oxidants (T-REX) and its genome-wide variant (G-REX)] were developed as a gateway to address these limitations. These protocols offer a new ability to both profile kinetically privileged sensors (KPSs) of RES at a systems level (G-REX£¿ profiling) and monitor signaling responses at the sensor protein-of-interest (POI)-specific level (T-REX£¿ delivery) with high spatiotemporal resolution. REX technologies are compatible with several model systems and are built on a HaloTag-targetable small-molecule photocaged precursor to a native RES
%U https://www.cell.com/trends/biochemical-sciences/fulltext/S0968-0004(19)30006-4