%0 Journal Article
%T Temporal Change of Extracellular Matrix during Vein Arterialization Remodeling in Rats
%A Ayumi Aurea Miyakawa
%A Jose Eduardo Krieger
%A Julliana Carvalho Campos
%A Monica Nunes Bizerra
%A Nubia Esteban Duarte
%A Thais Gir£¿o-Silva
%A Vin¨ªcius Bassaneze
%J -
%D 2019
%R https://doi.org/10.3390/jcdd6010007
%X Abstract The global expression profile of the arterialized rat jugular vein was established to identify candidate genes and cellular pathways underlying the remodeling process. The arterialized jugular vein was analyzed on days 3 and 28 post-surgery and compared with the normal jugular vein and carotid artery. A gene array platform detected 9846 genes in all samples. A heatmap analysis uncovered patterns of gene expression showing that the arterialized vein underwent a partial transition from vein to artery from day 3 to 28 post-surgery. The same pattern was verified for 1845 key differentially expressed genes by performing a pairwise comparison of the jugular vein with the other groups. Interestingly, hierarchical clustering of 60 genes with altered expression on day 3 and day 28 displayed an expression pattern similar to that of the carotid artery. Enrichment analysis results and the network relationship among genes modulated during vein arterialization showed that collagen might play a role in the early remodeling process. Indeed, the total collagen content was increased, with the augmented expression of collagen I, collagen IV, and collagen V in arterialized veins. Additionally, there was an increase in the expression of versican and Thy-1 and a decrease in the expression of biglycan and ¦Â1-integrin. Overall, we provide evidence that vein arterialization remodeling is accompanied by consistent patterns of gene expression and that collagen may be an essential element underlying extracellular matrix changes that support the increased vascular wall stress of the new hemodynamic environment. View Full-Tex
%K vein arterialization
%K microarray
%K extracellular matrix
%K collagens
%U https://www.mdpi.com/2308-3425/6/1/7