%0 Journal Article
%T Terminomics Methodologies and the Completeness of Reductive Dimethylation: A Meta-Analysis of Publicly Available Datasets
%A Iain J. Berry
%A Mariella Hurtado Silva
%A Matthew P. Padula
%A Natalie Strange
%A Steven P. Djordjevic
%J -
%D 2019
%R https://doi.org/10.3390/proteomes7020011
%X Abstract Methods for analyzing the terminal sequences of proteins have been refined over the previous decade; however, few studies have evaluated the quality of the data that have been produced from those methodologies. While performing global N-terminal labelling on bacteria, we observed that the labelling was not complete and investigated whether this was a common occurrence. We assessed the completeness of labelling in a selection of existing, publicly available N-terminomics datasets and empirically determined that amine-based labelling chemistry does not achieve complete labelling and potentially has issues with labelling amine groups at sequence-specific residues. This finding led us to conduct a thorough review of the historical literature that showed that this is not an unexpected finding, with numerous publications reporting incomplete labelling. These findings have implications for the quantitation of N-terminal peptides and the biological interpretations of these data. View Full-Tex
%K terminomics
%K mass spectrometry
%K amine labelling
%U https://www.mdpi.com/2227-7382/7/2/11