%0 Journal Article
%T Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
%A Heikki Ruskoaho
%A Hong Wang
%A Ilkka Tikkanen
%A Jere Paavola
%A Karri Kalervo
%A Katariina Immonen
%A Mika Laine
%A P£żivi Lakkisto
%A Riikka Kosonen
%A Sanni Perttunen
%A Suneeta Narumanchi
%J -
%D 2019
%R https://doi.org/10.3390/jcdd6020016
%X Abstract The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration. View Full-Tex
%K let-7c
%K miRNAs
%K epicardial cells
%K cardiac regeneration
%K proliferating cardiomyocytes
%K fibrin
%U https://www.mdpi.com/2308-3425/6/2/16