%0 Journal Article
%T Cytotoxicity of Contrast Media Iohexol on IL-1beta stimulated Bovine Chondrocytes
%A G. HONG
%A G. KIM
%A Y. HWANG
%J -
%D 2018
%R http://dx.doi.org/10.12681/jhvms.16394 http://dx.doi.org/10.12681/jhvms.16394
%X The aim of this study was to determine the cytotoxic and metabolic effects of iohexol on cultured bovine chondrocytes in clinical dose. Chondrocytes were exposed to 50%, 25% and 12.5% iohexol and 50% mannitol for 2 hours. Cell proliferation, apoptosis and necrosis were analyzed. Real time PCR was performed for aggrecan, collagen type I and II gene expression. Cells in alginate beads stimulated by interleukin-1¦Â (IL-1¦Â) were analyzed for cytotoxicity. MTT assay showed that 50% iohexol inhibited the proliferation of cells at 2 hours culture period. Propidium iodide results showed significantly higher dead cells at 50% iohexol compared to control, however PCR results revealed that chondrogenic gene were not affected. Cells in alginate beads stimulated with IL-1¦Â showed significantly higher percentage of dead cells at 50% iohexol exposure (p<0.05). These results suggested that iohexol has a cytotoxicity on chondrocytes and this cytotoxic effect possibly increased in inflammatory joint diseases
%K Alginate beads
%K Chondrocytes
%K Chondrogenic gene
%K Cytotoxicity
%K Iohexol
%U https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/16394