%0 Journal Article
%T Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat
%A A. Kustantinah
%A D. Maharani
%A L. Latifah
%A T. Hartatik
%J -
%D 2018
%R 10.14710/jitaa.43.4.343-351
%X Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5¡¯-TCGGGCGTCTTGTTCATCAT-3¡¯ and R: 5¡¯-CAAGACTGGGCACTGCTTCA-3¡¯). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0¡À1.7; AG=11.2¡À3.6; GG=9.2¡À3.9), weaning body length (WBL; AA=46.8¡À2.4; AG=46.3¡À6.0; GG=43.8¡À5.3) and weaning chest circumference (WCC; AA=50.9¡À4.0; AG=50.7¡À4.6; GG=47.1¡À7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6¡À2.1; CT=12.5¡À2.5) and average daily gain (ADG; CC=81.2¡À21.8; CT=79.3¡À26.2) than TT genotype (WW=10.6¡À3.1; ADG=58.3¡À30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat
%K Bligon
%K goat
%K MC4R
%K SNP
%K growth traits
%K association
%U https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304