%0 Journal Article %T A Novel Urine Method for the Diagnosis of Active Tuberculosis by Immunoassay for the Detection of ESAT-6 Using Hydrogel Nanoparticles in HIV Patients %A Luz Huaroto %A Raquel Mugruza %A Marinelly Roca %A Ricardo Antiparra %A CЈІsar Ticona %A Patricia Sheen %A Eduardo Ticona %A Robert H. Gillman %A Alessandra Luchini %A Lance Liotta %J Journal of Tuberculosis Research %P 73-84 %@ 2329-8448 %D 2021 %I Scientific Research Publishing %R 10.4236/jtr.2021.92006 %X Background: In patients with HIV, conventional tests are of low sensitivity; therefore, a new diagnostic test with hydrogel nanoparticles with reactive blue dye is proposed, which allows the capture, conservation and concentration of ESAT-6 in urine. NIPAs are copolymers that capture low molecular weight proteins and protect against enzymatic degradation. Using an immunoassay, it is possible to detect and quantify ESAT-6 present in urine as a possible marker of active TB. Design/Methods: Study in Lima, Peru, HIV+ participants, Ён18 years with and without tuberculosis (TB). Smear microscopy, culture in solid medium and urine immunoassay were performed. The reference was the diagnosis of TB by radiological or clinical microbiological criteria (indication for TB treatment). There were 2 preanalytical processes: untreated and treated urine (centrifuged, heated), then incubation with NIPAm. After washing, elution, sonication, heat and centrifugation, the final eluate was obtained. This was placed on nitrocellulose membranes, which by means of fixation and incubation processes with anti-ESAT-6 and anti-IgG antibodies, revelation with C-DiGit&reg Blot Scanner and FluorChem R FR0001. Calibration curves were included in the membranes, density was measured using Image J software. ROC curves, sensitivity and specificity were obtained. Results: The result by groups was HIV+ patient: ROC: 0.75, cut-off point Ён24.06 ng/ml, sensitivity 76.32%, specificity 68.89%, patients Ём200 cells CD4 mm³/ml, ROC: 0.78, cutoff point Ён26.20 ng/ml, sensitivity 75.86%, specificity 71.88%, patients >200 CD4 %K Tuberculosis %K HIV+ %K Hydrogel Nanoparticles and ESAT-6 %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=108640