%0 Journal Article
%T Potential of Two <i>Metarhizium anisopliae</i> (Clavicipitaceae) Isolates for Biological Control of <i>Diatraea saccharalis</i> (Lepidoptera: Crambidae) Eggs
%A Camila Vieira da Silva
%A Bruno Vinicius Daquila
%A Larissa Carla Lauer Schneider
%A Ronaldo Roberto Tait Caleffe
%A Julio Cesar Polonio
%A Daniele Araujo Canazart
%A Satiko Nanya
%A Helio Conte
%J Advances in Entomology
%P 63-76
%@ 2331-2017
%D 2022
%I Scientific Research Publishing
%R 10.4236/ae.2022.101005
%X Chemical
pesticides tend to accumulate in soil, resulting in human and environmental
health risks. Hence, alternative methodologies involving chemical pesticides
are beneficial for the control of agricultural pests. </span></span><span><span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">&lt;i&gt;</span><i><span style=\"font-family:Verdana;\">Metarhizium
anisopliae&lt;/i&gt;</span></i> </span></span></span><span><span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">is an
entomopathogenic fungus that acts on different developmental stages of pest
insects such as &lt;i&gt;</span><i><span style=\"font-family:Verdana;\">Diatraea
saccharalis&lt;/i&gt;</span></i><span style=\"font-family:Verdana;\">, a holometabolic lepidopteran with high potential for
infestation in sugarcane crops. The present study evaluated the biocontrol
effect of &lt;i&gt;</span><i><span style=\"font-family:Verdana;\">M. anisopliae&lt;/i&gt;</span></i><span style=\"font-family:Verdana;\"> isolates MT and E9 on &lt;i&gt;</span><i><span style=\"font-family:Verdana;\">D.
saccharalis&lt;/i&gt;</span></i><span style=\"font-family:Verdana;\"> eggs at different ages by investigating the external
and internal morphological alterations in treated eggs. Conidial suspensions of
&lt;i&gt;</span><i><span style=\"font-family:Verdana;\">M. anisopliae&lt;/i&gt;</span></i><span style=\"font-family:Verdana;\"> isolated from MT and E9 at concentrations of 10&lt;sup&gt;</span><sup><span style=\"font-family:Verdana;\">7</span></sup><span style=\"font-family:Verdana;\">&lt;/sup&gt;
conidia/mL were applied to eggs of &lt;i&gt;</span><i><span style=\"font-family:Verdana;\">D.
saccharalis&lt;/i&gt;</span></i><span style=\"font-family:Verdana;\"> aged 0, 24, 48, 72, 96 and 120 h. The eggs were
observed every 24 h during development (0 h to 144 h). Samples were collected
for observational, histological, and ultrastructural analyses. We found that
the MT isolate caused 100% inviability of eggs aged 0 - 72 h, 144 h after the
bioassays, while the effect of the E9 isolate varied between 49.40% and 93.75%.
Melanization was observed on the periphery of the eggs 24 h after the
bioassays. Fungal hyphae developed 48 h after bioassays, crossed the egg
chorion, and dispersed through the yolk region, inhibiting embryonic
development. After 72 h, hyphae and conidiophores were observed on the eggs,
which persisted for 144 h. In sum, &lt;i&gt;</span><i><span style=\"font-family:Verdana;\">M.
anisopliae&lt;/i&gt;</span></i><span style=\"font-family:Verdana;\"> MT isolate can be
%K Entomopathogen
%K Fungi
%K Management
%K Sugarcane
%K Sustainability
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=113673