%0 Journal Article
%T Molecular Detection of Carbapenemase Genes in Extensive Drug Resistant <i>Acinetobacter baumannii</i> Clinical Isolates from ICU Patients, Khartoum
%A Shirehan M. Ibrahim
%A Elamin M. Ibrahim
%A Omer A. Ibrahim
%A Omnia M. Hamid
%A Hassan A. Alaziz
%J Open Journal of Medical Microbiology
%P 38-48
%@ 2165-3380
%D 2022
%I Scientific Research Publishing
%R 10.4236/ojmm.2022.121004
%X <b><span style=\"font-family:Verdana;\">Background:</span></b><span style=\"font-family:Verdana;\"> The emergence of carbapenemase producing</span><span style=\"font-family:&quot;\"> </span><i><span style=\"font-family:Verdana;\">Acinetobacter baumannii</span></i><span style=\"font-family:&quot;\"> </span><span style=\"font-family:Verdana;\">is</span><span style=\"font-family:Verdana;\"> increasingly reported nowadays and constitute</span><span style=\"font-family:Verdana;\">s</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> a
major problem to the intensive care unit (ICU) patients with notable
extensive-drug resistance ability. The study investigates carbapenemase
producing </span><i><span style=\"font-family:Verdana;\">A. baumannii</span></i><span style=\"font-family:Verdana;\"> strains exhibiting an extensively drug-resistant
(XDR) phenotype, isolated from ICU patients in Khartoum. </span><b><span style=\"font-family:Verdana;\">Methods:</span></b><span style=\"font-family:Verdana;\"> A
total of 100 nonduplicate Gram-negative coccobacilli strains were obtained from
microbiology laboratory of ICU patients</span></span><span style=\"font-family:Verdana;\">กฏ</span><span style=\"font-family:Verdana;\"> clinical isolates. Molecular identification </span><span style=\"font-family:Verdana;\">of </span><i><span style=\"font-family:Verdana;\">A. baumannii</span></i><span style=\"font-family:Verdana;\"> was performed by targeting 16S rRNA gene using specifically designed
primers. Then, XDR strains were determined by susceptibility testing (disc
diffusion). For detection of carbapenemase genes Polymerase chain reaction
(PCR) was carried out.</span><span style=\"font-family:&quot;\"> </span><b><span style=\"font-family:Verdana;\">Result:</span></b><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> Of 100 ICU clinical isolates, 38 (38.0%) was confirmed </span><i><span style=\"font-family:Verdana;\">A. bauman</span></i></span><i><span style=\"font-family:Verdana;\">n</span></i><i><span style=\"font-family:Verdana;\">ii</span></i><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> strains, those strains showed 100% carbapenem resistance and 60.5% extensive
drug resistance to the antibiotics tested. The frequency of carbapenemase
producer was 57.9% (22/38) of carbapenem resistance </span><i><span style=\"font-family:Verdana;\">A. baumannii</span></i><span style=\"font-family:Verdana;\"> (CRAB). The most common carbapenemase associated with
resistance was blaOXA gene followed by blaNDM and blaGES
%K Carbapenem Producing &lt
%K i&gt
%K Acinetobacter baumannii&lt
%K /i&gt
%K (CPAB)
%K Intensive Care Unit (ICU) Patients
%K Extensive Drug-Resistant (XDR)
%K Colistin
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=116111