%0 Journal Article
%T <i>SLCO1B1</i>, <i>NAT2</i> Polymorphisms and Pharmacokinetic Variability of Rifampicin and Isoniazid in Tuberculosis Patients from Sub-Saharan Africa
%A Sekossounon Sanni
%A Ablo Prudence Wachinou
%A Corinne Simone Colette Merle
%A Lamine Baba-Moussa
%A Dissou Affolabi
%J Journal of Tuberculosis Research
%P 111-123
%@ 2329-8448
%D 2022
%I Scientific Research Publishing
%R 10.4236/jtr.2022.103009
%X SLCO1B1 and NAT2 polymorphisms have been associated with the variability of Rifampicin and Isoniazid pharmacokinetic
(PK). The objective of this study was to identify in African patients with
tuberculosis (TB) or TB/HIV co-infection,
the SLCO1B1 and NAT2
polymorphisms, associated with the variability of Rifampicin and
Isoniazid pharmacokinetic. TB or TB/HIV co-infected patients from Benin,
Guinea, Senegal, and South Africa were included in this study. The blood
samples collected were stored at -80˚C until DNA extractions. The DNA extracts were then frozen at -80˚C after quality control. Double stranded DNA of
the samples were quantified using a fluorimetric method to select suitable
samples for the preparation of 96-well microplates, containing 100 ¦Ěl of DNA
extract per well at the concentration of 20 ng/¦Ěl.
Illumina HumanOmniExpress-24 v1.2 microarray genotyping was performed by an external vendor. The genotyping data
were analyzed and the polymorphisms with a call rate < 95% or presenting a
departure from the