%0 Journal Article
%T Effects of Inoculum Size of <i>Afifella marina</i> in the Bioprocessing of <i>Sargassum</i> spp. Meal
%A Sujjat Al Azad
%A Mohammad Tamrin Bin Mohamad Lal
%J Advances in Biological Chemistry
%P 185-195
%@ 2162-2191
%D 2022
%I Scientific Research Publishing
%R 10.4236/abc.2022.126016
%X Brown seaweed, <em>Sargassum</em> spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as <em>Lactobacillus</em>, <em>Bacillus</em> or <em>Streptococcus</em>, are improved with different inoculum sizes. Purple non-sulphur bacterium, <em>Afifella marina</em> could be one of the alternative and potential candidates. This study aims to determine the optimum inoculum level and period for the improvement of nutritional values in <em>Sargassum polycystum</em> meal. <em>Sargassum polycystum</em> was collected from the Sepanggar Bay. Dried seaweed was grinded into fine particles (&lt;400 ¦Ìm). Inoculum sizes of 10% (v/v), 20% (v/v) and 30% (v/v) of <em>A. marina</em> were used with 20 g <em>Sargassum polycystum </em>powder in one liter bottle with autoclaved 112 synthetic media. Bottles were incubated at 2500 lux light intensity at 30<span style=\"white-space:nowrap;\">&amp;#730;</span>C ¡À 1<span style=\"white-space:nowrap;\">&amp;#730;</span>C for 8 days. Every two days destructive samples were taken for the determination of nutritional values of bioprocessed seaweed meal. The measured analytical parameters were crude protein (%), crude ash (%), crude fiber (%) and crude lipids (%). Nutritional value of processed seaweed meal powder with <em>A. marina</em> has improved with the increase of inoculum size. The crude protein percentage of 14.70% + 0.40% was significantly high (p &lt; 0.05) with 30% (v/v) inoculum size and obtained on the 8<sup>th</sup> of bioprocessing product. Consistent decrease in crude fiber values was observed with increase of inoculum sizes, as 19.34% of crude fiber decrement was determined with 30% (v/v) inoculum level on 6<sup>th</sup> day. The performance of crude lipids was observed insignificant in all inoculum levels. No significant differences (p &gt; 0.05) were observed among the values of crude protein, ash, lipids and fiber within 6<sup>th</sup> and 8<sup>th</sup> day. <em>A. mariana</em> with 30% (v/v) inoculum sizes on 6<sup>th</sup> of day has capability in improving the nutritional values of <em>Sargassum polycystum</em> seaweed meal during bioprocessing.
%K &lt
%K i&gt
%K Afiffella mariana
%K Sargassum&lt
%K /i&gt
%K Nutritional Values
%K Inoculum Sizes and Period
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=121533