%0 Journal Article
%T Comparative Performance of Microscopy and Nested PCR for the Detection of <i>Cryptosporidium</i> Species in Patients Living with HIV/AIDS in Abidjan (Côte d¡¯Ivoire)
%A N¡¯Docho Assi Fiacre-Tanguy
%A Gon¨¦d¨¦l¨¦ Bi Sery Ernest
%A Tuo Karim
%A Bial¨¦ Marina Danielle Datcho-Gouenet Ayaud
%A N¡¯Guessan Tiacoh Landry
%A Koffi Attoungbr¨¦ Cl¨¦ment
%A Koffi David
%A Ako Ako Aristide B¨¦renger
%A Vanga Bosson Abo Henriette
%A Tour¨¦ Offianan Andr¨¦
%J American Journal of Molecular Biology
%P 18-31
%@ 2161-6663
%D 2023
%I Scientific Research Publishing
%R 10.4236/ajmb.2023.131002
%X <em>Cryptosporidium</em> spp. infection is one of the causes of diarrhea in people living with HIV/AIDS. The objective of this study is to compare the sensitivity of microscopy and molecular biology to determine the prevalence of <em>Cryptosporidium</em> spp. in Patients Living With HIV (PLWH). This is a descriptive cross-sectional study conducted in three care centers for people living with HIV/AIDS in Abidjan. It took place from November 2018 to March 2020. Sociodemographic data were obtained via a questionnaire. Stool and blood samples were collected and analyzed for microscopy and Nested PCR detection of <em>Cryptosporidium</em> spp. Blood samples were analyzed for CD4+ count. A total of 363 stool samples were collected from the three sites. Individuals aged 40 - 50 years (36.52%) were most likely to participate in the study. HIV Type 1 accounted for 86.22% of the study population. The samples collected consisted of 47.65% diarrheal stool. Microscopic examination of the stool yielded a prevalence of 3.86% for <em>Cryptosporidium</em> spp. while the prevalence was 3.96% with molecular identification. No statistically significant difference was observed between these two prevalences (¦Ö<sup>2</sup> = 0.26; p = 0.609). CD4+ count was the factor associated with <em>Cryptosporidium</em> spp. infection for both microscopy (OR = 0.887, p = 0.001) and PCR (OR = 0.896, p = 0.001). This study demonstrated that Nested PCR improves the detection of <em>Cryptosporidium</em> spp. in patient diagnosis.
%K &lt
%K i&gt
%K Cryptosporidium&lt
%K /i&gt
%K PCR
%K Microscopy
%K PLHIV
%K C&amp
%K #244
%K te D¡¯Ivoire
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=121985