%0 Journal Article
%T 基于网络药理学的绞股蓝治疗急性肺损伤疗效分析<br>Analysis of the Efficacy of Gynostemias pentaphyllum in the Treatment of Acute Lung Injury Based on Network Pharmacology
%A 吕冠平
%A 赵敏
%J Hans Journal of Biomedicine
%P 20-34
%@ 2161-8984
%D 2023
%I Hans Publishing
%R 10.12677/HJBM.2023.131003
%X 目的：基于网络药理学研究绞股蓝治疗急性肺损伤(acute lung injury, ALI)的有效成分和潜在靶点。方法：从中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP)中获取绞股蓝的活性成分、相关靶点和相关靶基因。同时，通过OMIM数据库、GeneCards数据库和Therapeutic Target数据库获取ALI靶基因信息。文氏图用于显示绞股蓝和ALI的共同靶点。使用Cytoscape 3.7.2构建药物成分靶点疾病网络图，并从STRING数据库获得蛋白–蛋白相互作用(Protein-Protein Interaction Networks, PPI)网络。同时，使用生物信息学网站平台进行基因本体富集分析和KEGG (Kyoto Encyclopedia of Genes and Genomes)通路富集分析以揭示该机制。结果：结果分为三个阵营：成分、靶点和途径。在成分方面，发现绞股蓝的15种活性成分在细胞膜、细胞质和细胞核中具有生物活性，其中槲皮素、鼠李素和异岩藻甾醇是主要活性成分。共发现155个靶点，其中134个主要靶点和ALI共同拥有(尤其是AKT1 (Protein Kinase Bα)、TP53 (tumor protein p53)、TNF (tumor necrosis factor)、IL-6 (Interleukin-6)、VEGFA (Vascular endothelial growth factor A)、CASP3 (Caspase-3)、IL-1B (Interleukin-1B)、HIF-1A (hypoxia inducible factor-1A)、EGFR (Epidermal growth factor receptor)和PTGS2 (Prostaglandin-Endoperoxide Synthase 2))有助于ALI的治疗。此外，绞股蓝治疗ALI的主要途径是PI3K-Akt (Phosphatidylinositol-3-kinase)信号通路、脂质和动脉粥样硬化、糖尿病并发症中的AGE-RAGE (advanced glycosylation end products-receptor of AGEs)信号通路、HIF-1信号通路、肿瘤和感染相关通路。结论：由于绞股蓝的多组分、多靶点和多通道功能，本研究通过网络药理学初步揭示了绞股蓝治疗ALI的潜在调节网络。为后续的实验研究和临床应用提供了理论依据。<br />
Objective: To study the active components and potential targets of Gynostemma pentaphyllum in the treatment of acute lung injury (ALI) based on network pharmacology. Methods: The active components, related targets and related target genes of Gynostemma pentaphyllum were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). At the same time, ALI target gene information is obtained through OMIM database, GeneCards database and Therapeutic Target database. Venn diagram is used to show the common targets of Gynostemma pentaphyllum and ALI. Use Cytoscape 3.7.2 to construct the target disease network diagram of drug components, and obtain the protein-protein interaction networks (PPI) network from STRING database. At the same time, the bioinformatics website platform was used for gene ontology enrichment analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis to reveal the mechanism. Results: The results were divided into three camps: component, target and pathway. In terms of components, it was found that 15 active components of Gynostemma pentaphyllum had biological activities in the cell membrane, cytoplasm and nucleus, among which quercetin, rhamnolicin and isofucosterol were the main active components. A total of 155 targets were found, 134 of which were jointly owned by ALI (especially AKT1 (Protein Kinase Bα), TP53 (tumor protein p53), TNF (tumor neurosis
%K 网络药理学，绞股蓝，急性肺损伤，活性成分，机制<br>Network Pharmacology
%K Gynostemma pentaphyllum
%K Acute Lung Injury
%K Active Components
%K Mechanism
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=60317