%0 Journal Article
%T Isolation of Picocyanobacteria (Order <i>Synechococcales</i>) and Occurrence of the Cyanotoxin Anatoxin-A in a Shallow Mesotrophic Pond
%A Nancy J. Leland
%A Kimberley Crocker Pearson
%A Marty K. Burke
%A Jeffrey T. Miller
%A Alison Watts
%A James F. Haney
%J Journal of Water Resource and Protection
%P 299-314
%@ 1945-3108
%D 2023
%I Scientific Research Publishing
%R 10.4236/jwarp.2023.156017
%X We have verified the use of a serial filtration method to isolate picocyanobacteria for analysis. We used eDNA metabarcoding to confirm the picocyanobacteria as members of the Order <em>Synechococcales</em>, Genus <em>Cyanobium</em>, specifically <em>Cyanobium </em>6307. Fluorometric analysis using accessory pigments phycocyanin and phycoerythrin described periods of excess biomass, where the net growth rate model confirmed these conditions. The total anatoxin-a concentrations in the picocyanobacterial sample ranged from 0.0074 - 6.41 ¦Ìg<span style=\"text-wrap:nowrap;\">&amp;middot;</span>L<sup>-1</sup> representing a 40-fold difference over the entire sampling season. Sampling frequency of every three days appeared to be an important factor in capturing these changes in anatoxin-a concentration. During a period of excess biomass, we were able to establish a linear correlation between cyanobacterial biomass and Anatoxin-a concentrations.
%K Picocyanobacteria
%K Anatoxin-a
%K eDNA
%K Phycocyanin
%K Phycoerythrin
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=125865