%0 Journal Article
%T Validation of High Performance Liquid Chromatography with Fluorescence  Detector Methods for Determination  of Aflatoxins in Different Food and  Animal Feed Samples
%A Salah Eldeen Abass Ali Ahmed
%A Abdalla Ahmed Elbashir
%J Journal of Analytical Sciences, Methods and Instrumentation
%P 1-11
%@ 2164-2753
%D 2023
%I Scientific Research Publishing
%R 10.4236/jasmi.2023.131001
%X Method validation for quantitative analysis of aflatoxins, AFB1, AFB2,
AFG1 and AFG2 in sorghum, peanut butter, groundnut and animals feed is
presented. Aflatoxins were extracted with a mixture of methanol: acetonitrile:
water (60:30:10) and cleaned with Alfa test<span><span><span style=\"font-family:;\" \"=\"\"> </span></span></span><span><span><span style=\"font-family:;\" \"=\"\">IAC
chromatography before analysis by High Performance Liquid Chromatography
coupled with fluorescence detection (HPLC-FLD) by adopting an isocratic
chromatographic system using a mobile phase consisting acetic acid:
acetonitrile: methanol (59:14:27), the separation of the four aflatoxins was
achieved in less than 15 minutes. Calibration curves were linear over the
concentration range from 2</span></span></span><span><span><span style=\"font-family:;\" \"=\"\"> - </span></span></span><span><span><span style=\"font-family:;\" \"=\"\">18 ng/mL for AFB1 and AFG1, 0.4</span></span></span><span><span><span style=\"font-family:;\" \"=\"\"> - </span></span></span><span><span><span style=\"font-family:;\" \"=\"\">3.6 ng/mL for AFB2 and AFG2, respectively. The LOD and LOQ in spiked
samples were found to be 0.02 and 0.05 ¦Ìg/kg for both AFB1 and AFG1, 0.01 and
0.03 ¦Ìg/kg for both AFB2 and AFG2. The mean recovery values were in range from
84.2</span></span></span><span><span><span style=\"font-family:;\" \"=\"\">%</span></span></span><span><span><span style=\"font-family:;\" \"=\"\"> to 96.9% was obtained. Five samples were found to be contaminated with
aflatoxins and the total aflatoxins ranged from 0.02 to</span></span></span><span><span><span style=\"font-family:;\" \"=\"\"> </span></span></span><span><span><span style=\"font-family:;\" \"=\"\">3.26
¦Ìg/kg were obtained. Nineteen different samples were found to be contaminated
with aflatoxins; the total aflatoxins ranged from 0.27 to</span></span></span><span><span><span style=\"font-family:;\" \"=\"\"> </span></span></span><span><span><span style=\"font-family:;\" \"=\"\">10.48
¦Ìg/kg were obtained. The highest total aflatoxins value was obtained in animal
feeds.</span></span></span>
%K Aflatoxins
%K HPLC-FLD
%K Peanut
%K Peanut Butter
%K Sorghum and Animal Feeds
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=126216