%0 Journal Article
%T Isolation and Identification Methods for Oral <i>Klebsiella pneumoniae</i> Involved in Onset of Inflammatory Bowel Disease
%A Osamu Tsuzukibashi
%A Akira Fukatsu
%A Hiroshi Yamamoto
%A Yuji Takahashi
%A Keisuke Usuda
%A Mana Fuchigami
%A Chiaki Komine
%A Satoshi Uchibori
%A Koji Umezawa
%A Sachiyo Hayashi
%A Takashi Asano
%A Masanobu Wakami
%A Hiroshi Murakami
%A Taira Kobayashi
%A Masahiko Fukumoto
%J Open Journal of Stomatology
%P 197-211
%@ 2160-8717
%D 2023
%I Scientific Research Publishing
%R 10.4236/ojst.2023.137017
%X <strong>Purpose:</strong> Recently, it was reported that <em>Klebsiella pneumoniae</em> is related to the onset of inflammatory bowel disease including the Crohn disease. It was frequently reported that<em> K. pneumoniae</em> was detected in human oral cavities. Regrettably, it currently remains unclear whether <em>K. pneumoniae</em> is part of the normal oral flora. The aim of this study was to establish the isolation and identification methods for <em>K. pneumoniae</em> from human oral cavities, and investigate its transmission pattern. <strong>Methods:</strong> A selective medium, OKPSM, for the isolation of <em>K. pneumoniae</em> from oral cavities was developed in this study. Also, PCR primer for the identification and detection at subspecies level of <em>K. pneumoniae</em> was designed. <strong>Results: </strong>OKPSM and PCR method using the primers designed in this study were useful for the isolation and identification of <em>K. pneumoniae</em> from human oral cavities. <em>K. pneumoniae</em> subsp. <em>pneumoniae</em> was detected at 10.0% in 30 saliva samples. On the other hand, <em>K. pneumoniae</em> subsp. <em>ozaenae</em> and<em> K. pneumoniae</em> subsp. rhinoscleromatis were detected from no sample. Moreover, <em>K. pneumoniae</em> subsp. <em>pneumoniae</em> isolates from same subject at 0 month and after 3 months showed same genotypes on AP-PCR using OPA-07 primer. <strong>Conclusion: </strong>These results indicated that human oral cavities were not suitable for the habitat of <em>K. pneumoniae</em>.
%K &lt
%K i&gt
%K Klebsiella pneumoniae&lt
%K /i&gt
%K Selective Medium
%K Oral Cavity
%K Multiplex PCR
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=126273