%0 Journal Article
%T Characterization of Mycobacterium Species Circulating among Tuberculosis Patients in Bayelsa State, Nigeria
%A Smart Enoch Amala
%A Goodluck Silas
%A Amba Ollor Ollor
%A Vivian Nkemkanma Agi
%J Journal of Tuberculosis Research
%P 95-108
%@ 2329-8448
%D 2023
%I Scientific Research Publishing
%R 10.4236/jtr.2023.113010
%X <span style=\"font-family:Verdana;\"><b><span style=\"font-family:Verdana;\">Introduction: </span></b></span><span><span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">Tuberculosis is caused by infection with </span><i><span style=\"font-family:Verdana;\">Mycobacterium tuberculosis</span></i><span style=\"font-family:Verdana;\">. Looking at
the evolution of the bacterium gene due to mutation is crucial to identify
species</span><i> </i><span style=\"font-family:Verdana;\">circulating among patients in
an area. WHO speculated that tuberculosis is caused by </span><i><span style=\"font-family:Verdana;\">M</span></i><span style=\"font-family:Verdana;\">.</span><i><span style=\"font-family:Verdana;\"> tuberculosis </span></i><span style=\"font-family:Verdana;\">(MTB),
but identification of the strains of MTB circulating in a particular area is
important for the management of MTB and to identify pulmonary infections caused
by non-tuberculosis mycobacterium. Contact tracing of drug resistant MTB in
circulation in an area is also an important procedure of MTB therapeutic
choice. </span><b><span style=\"font-family:Verdana;\">Aim: </span></b><span style=\"font-family:Verdana;\">This study aimed to isolate and identify </span><i><span style=\"font-family:Verdana;\">Mycobacterium</span></i><span style=\"font-family:Verdana;\"> species</span><i> </i><span style=\"font-family:Verdana;\">circulating in Bayelsa State, Nigeria. </span><b><span style=\"font-family:Verdana;\">Materials
and Methods:</span></b><span style=\"font-family:Verdana;\"> A total of 102 sputum samples collected from MTB patients were
cultured in Lowenstein Jensen (LJ) solid media. Isolates on LJ media were
confirmed using Zeihl Nelseen staining method for AFB and Standard Diagnosis
Bioline TB Ag MPT64 Rapid test kit. The 16s rRNA gene amplification, agarose
gel electrophoresis, and gene sequencing were conducted. Phylogenic analysis
and evolutional distances of the strains are computed using the Juke-cantor
method. </span><b><span style=\"font-family:Verdana;\">Result: </span></b><span style=\"font-family:Verdana;\">Out of 102 sputum samples examined 15</span></span></span></span><span><span><span style=\"font-family:&quot;\"> </span></span></span><span><span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">(14.7%)
had growth of </span><i><span style=\"font-family:Verdana;\">Mycobacterium</span></i><span style=\"font-family:Verdana;\"> species
(AFB positive). The extracted DNA of MTB amplified on agarose gel
electrophoresis aligned horizontally at
%K Tuberculosis
%K Non-Tuberculosis
%K Strains
%K Circulating
%K Bayelsa
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=126729