%0 Journal Article
%T Resistance of <i>Klebsiella</i> to Imipenem by Production of Carbapenemase Gene <i>bla</i><i><sub>IMP</sub></i> at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso
%A Blandine Ou¨¦draogo
%A Abdoul Karim Ouattara
%A Amana M¨¨tuor Dabir¨¦
%A Rahimatou Yasmine Wendkuni Tiemtor¨¦
%A Serge Soug¨¦
%A Jacques Simpor¨¦
%J International Journal of Clinical Medicine
%P 347-356
%@ 2158-2882
%D 2023
%I Scientific Research Publishing
%R 10.4236/ijcm.2023.148030
%X <strong>Objective: </strong>Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of <em>¦Â</em>-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene <em>bla</em><sub><em>IMP</em></sub> in the genus <em>Klebsiella</em> at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. <strong>Methods: </strong>The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. <strong>Results: </strong>The antibiotic susceptibility test showed high resistance of the 17 <em>Klebsiella</em> isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with<em> bla</em><sub><em>IMP</em></sub>-type gene. <strong>Conclusion:</strong> <em>Klebsiella</em> is a well-known bacterium in clinical practice. The present study demonstrated the <em>bla</em><em><sub>IMP</sub></em>-type gene in cephalosporin-resistant strains of<em> Klebsiella</em> at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.
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%K Klebsiella&lt
%K /i&gt
%K Resistance
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%K i&gt
%K bla&lt
%K /i&gt
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%K i&gt
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%K sub&gt
%K IMP&lt
%K /sub&gt
%K &lt
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%K Genes
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%K ¦Â&lt
%K /i&gt
%K -lactam
%K Burkina Faso
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=126851