%0 Journal Article
%T 展萼金丝桃化学成分及其抗氧化活性研究<br>Study on the Chemical Constituents from Hypericum lancasteri and Their Antioxidant Activities
%A 张潘潘
%A 周英
%A 梁嘉俊
%A 梁婷
%A 刘爽
%A 余欢
%A 魏鑫
%J Hans Journal of Medicinal Chemistry
%P 167-173
%@ 2331-8295
%D 2023
%I Hans Publishing
%R 10.12677/HJMCe.2023.113021
%X 目的：对展萼金丝桃(Hypericum lancasteri N. Robson)的化学成分进行分离鉴定；对其单体化合物进行抗氧化活性评价。方法：展萼金丝桃干燥地上部分经粉碎，95%甲醇回流提取得总提取物，使用硅胶、凝胶、MCI、ODS柱色谱、高效液相色谱(HPLC)等多种分离技术对总提取物进行分离纯化，结合核磁共振氢谱(1H NMR)、碳谱(13C NMR)以及质谱(MS)等解析手段，通过文献数据分析确定所得化合物结构。采用DPPH自由基清除法对化合物1-4进行抗氧化活性评价。结果：共分离得到3个xanthone类化合物和2个其他类化合物，分别鉴定为1,7-dihydroxyxanthone (1), 2-hydroxyxanthone (2), 2,3-二甲氧基口山酮(3), hyperdioxanes B (4) 和2-methyl-6-isopropyl-7-hydroxymethyl naphthalene (5)，化合物1-5均为首次从该植物中分离得到。化合物1-4在400 μmol/L浓度下的DPPH自由基清除率分别为11.44%，9.68%，11.04%，9.72%。结论：从展萼金丝桃地上部分分离得到5个单体化合物，均为首次从该植物中分离得到，丰富了该植物的物质基础，同时生物活性研究为展萼金丝桃在抗氧化活性方面的药物应用提供了线索。<br />
Objective: To isolate and identify the chemical composition of Hypericum lancasteri N. Robson; evaluate the antioxidant activity of its monomer compounds. Methods: The dried aerial part of H. lancasteri was crushed, and the total extract was extracted by 95% methanol reflux. The total extract was separated and purified by silica gel column chromatography, MCI column chromatography, ODS column chromatography, high performance liquid chromatography (HPLC) and other separation technologies. The structure of the isolated monomers was determined by 1H and 13C nuclear magnetic resonance (NMR) as well as mass spectrometry (MS). The antioxidant activity for compounds 1-4 was evaluated using DPPH radical scavenging method. Results: A total of three xanthones together with two other compounds were isolated and identified as 1,7-dihydroxyxanthone (1), 2-dihydroxyxanthone (2), 2,3-dimethoxyxanthone (3), hyperdioxanes B (4), and 2-methyl-6-iso- propyl-7-hydroxymethyl naphthalene (5) respectively. Compounds 1-5 were isolated from this plant for the first time. The DPPH radical scavenging rates of compounds 1-4 (400 μmol/L) were 11.44%, 9.68%, 11.04% and 9.72%, respectively. Conclusion: Five monomeric compounds were isolated from the aerial part of the H. lancasteri, all of which were the first to be isolated from the plant, enriching the material foundation of the plant. At the same time, biological activity research provided clues for the drug application of the plant in terms of antioxidant activity.
%K 展萼金丝桃，抗氧化活性，化学成分，DPPH，Xanthone<br>Hypericum lancasteri
%K Antioxidant Activity
%K Chemical Composition
%K DPPH
%K Xanthones
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=70281