%0 Journal Article
%T Biotechnological Assessment of <i>Suaeda arcuate</i> under <i>in Vitro</i> Conditions
%A Khulkar Khalbekova
%A Jamoliddin Ziyavitdinov
%J Advances in Bioscience and Biotechnology
%P 359-367
%@ 2156-8502
%D 2023
%I Scientific Research Publishing
%R 10.4236/abb.2023.148023
%X This article presents data on the introduction of <i>in
vitro</i> culture and microclonal propagation of plants identified in the group
of hyperhalophytes belonging to the species <i>Suaeda arcuata</i> <i>Bunge</i>. This study was carried
out to optimize the composition of nutrient media for the main stages of
reproduction <i>in vitro</i>, as well as studies on the rooting and adaptation
of regenerants for species of the genus <i>Suaeda</i> obtained from axillary or
apical buds, but more often from stem segments with a node. In this work, hormones
of the cytokinin and auxin series, or a combination of them, were added to the
nutrient environment for growth activation. The analyzing of microplants for
the content of soluble in water B vitamins was carried out. As a result of the
research, it was found that intact <i>Suaeda
arcuata</i> plants in their natural habitats produce a large amount of
soluble in water vitamins: riboflavin¡ª0.062% and thiamine up to 0.006%. And in
regenerated plants obtained on media without hormones, the content of vitamins
was: B2 0.053%, B1 0%, respectively, and with a combination of 1/2 MS + 1 mg/l
6-BAP + 0.3 mg/l IAA + 2, 4-D, the content of vitamins varied as follows:
riboflavin¡ª0.059%, folic acid¡ª0.030%, and thiamine was not detected. The
cultivation of regenerates on the environment 1/2 MS + 1 mg/l 6-BAP + 0.3 mg/l
IAA + 2,4-D showed the best effect on the growth of regenerants, created the
possibility of obtaining the maximum amount of biomass and accumulation of B
vitamins.
%K Aralkum
%K Hyperhalophyte
%K &lt
%K i&gt
%K In Vitro&lt
%K /i&gt
%K Explant
%K Sterilization
%K Seeds
%K Micropropagation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=127046